Evaluation of Cardiovascular Effects of Carvacrol in a D-(+)-Galactose-Induced aging Model

Aim: To evaluate the cardiovascular effect of carvacrol treatment in a D(+)galactose accelerated aging model, investigating effects on vascular reactivity, oxidative stress, and systolic blood pressure (SBP).
 Methodology: Eight-week-old male Wistar rats (Rattus norvegicus) were used for oral eight weeks. Organ baths reactivity studies (FEN, ACh, NPS), fluorescence microscopy to detect reactive oxygen species (ROS, using DHE probe), Tail-Cuff (SBP) measurements. Non-linear regression was create concentration-response curves. Emax denotes tissue's maximum response.
 Results: The aged showed significant increase intensity by probe compared CTL group (CTL=100 ± 3.6%, n=5 Dgal=167.7 7.9%, n=5, respectively). However, levels ROS carvacrol-treated groups significantly attenuated Dgal+C50 (138.8 4.5%, n=5) Dgal+C100 (130.0 5.5%, groups. animals Dgal presented hypertension through SBP (CTL=135.9 3.9 mmHg, n=6, Dgal=170.9 2.0 n=9, increased could be reversed with (Dgal+C50=137.9 2.7 Dgal+C100=124.6 8.2 respectively. On other hand, unable restore ACh-induced vasorelaxation found (Emax=100.0 3.9%), (Emax=84.9 4.4%), 4.4%) (Emax=82.1 6.2 %).
 Conclusion: Carvacrol shows protective antioxidant capable reducing animals, being an important tool promoting healthy aging.